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Voronezh State University - KBBE-2008-3-2-01 The atomno-force microscopy and others biophysical nanotechnologies in research of structurally functional properties of hydrolases

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Date of filling in 23.03.2009
Organization name Voronezh State University
Organization adress Universitetskaya pl., 1, Voronezh, 304006, Russia
Department/Unit Biological faculty / Chair of Biophysics and biotechnology
Contact person Kovaleva Tamara, Prof.
Phone +7-4732-208586; 208578
Fax +7-4732-208308
E-Mail tamara_kovaleva@inbox.ru
Web-site http://www.vsu.ru
Organization type
  • Research
  • Education
Special Programme
Theme
Food, Agriculture and Fisheries, and Biotechnology (KBBE)
Call identifier
Topic number KBBE-2008-3-2-01 The atomno-force microscopy and others biophysical nanotechnologies in research of structurally functional properties of hydrolases
Call Deadline
Short description of the organization (max 12 lines): -
Expertise offered The spatial structure of various hydrolases was investigated by methods of atomno-force microscopy, computer modelling, gel-chromatography, electrophoresis, IR-spectroscopy. Physical and chemical properties of enzyme preparations and kinetiko-thermodynamic aspects of hydrolysis reaction to polymeric substrate was studied. Working out of structurally functional models of imulinase, lipase, glucoamylase and also creation on their basis of heterogeneous biocatalysts to the prolonged action is planned.
Scientific keywords Inulinase Lipase Glucoamylase Atomic-force microscopy Oligomerous structure Heterogeneous biocatalysists
Publications on the topic (other references)
  • Computer_Aided Analysis of Spatial Structure of Some Hydrolytic Enzymes // V.G. Artyukhov, T.A. Kovaleva, O.M. Kozhokina, L.A. Bitutskaya, R.V. Dronov, O.D. Trofimova. – Biochemistryю – 2005. - Vol. 70, № 10, 2005. – P. 1086-1094.
  • Kinetic and thermodynamic aspects of polysaccharide hydrolysis by native and immobilized amylases // T.A. Kovaleva – Biophysics. – 2000. – Vol. 45, № 3. – P. 427-432.
  • Influence of urea, γ- and UV-radiation on physicochemical properties of the native and immobilized inulinase // T.A. Kovaleva, O.M. Kozhokina, O.D. Trofimova. – Radibiology. Radioecology. – 2000. – Vol. 40, №1. - С. 23-27.
  • Evaluation of the Number of Ionogenic Groups of Inulinase by Acid-Base Titration // T.A. Kovaleva, M.G. Holyavka, S.G. Rezvan, S.V. Kozhedub. - Bulletin of Experimental Biology and Medicine. – 2008. - Vol. 145, №. 6. P. 718-720.
  • Influence of UV irradiation on physicochemikal properties of immobilized inulinase Aspergillus awomari // O.D. Trofimova, O.M. Kozhokina, A.S. Taha - 8th IUBMB Conference. –Boston. - 2004. - p. 17
  • Investigation of kinetik and thermodynamic aspects of polymeric substrate hydrolisis by soluble and immobilization hydrolase // V.G. Artyukhov, T.A. Kovaleva, O.D. Trofimova, O.M. Kozhokina, A.S. Taha - Al-Mustansiria Journal of Science. - 2004. - №6. – P. 183-188.
  • Development of the heterogeneous biocatalyst for reaction of inulin hydrolysis on basis of immobilized inulinase preparation from Kluyveromyces marxianus // T.A. Kovaleva, M.G. Holyavka, A.S. Taha – Biotechnology [in Russian]. – 2007. - № 3. - С. 80-87.
Description of previous and present experience in International Cooperation -
Previous participation in EU’s Framework Programme projects