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Voronezh State University - Usage of new biological active molecule; Metabolomics

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Date of filling in 04.07.2008
Organization name Voronezh State University
Organization adress Universitetskaya pl., 1, Voronezh, 304006, Russia
Department/Unit Biology and Soil Science Faculty, Chair of Medical Biochemistry and Microbiology
Contact person Popova Tatyana, DSc, Professor, Head of Chair
Phone +7-4732-208278
Fax +7-4732-208755
E-Mail tpopova@bio.vsu.ru
Web-site http://www.vsu.ru
Organization type
  • Research
  • Education
Special Programme
Theme
Food, Agriculture and Fisheries, and Biotechnology (KBBE)
Call identifier
Topic number Usage of new biological active molecule; Metabolomics
Call Deadline
Short description of the organization (max 12 lines): The Voronezh State University (VSU) was established on the base of Yuryev University evacuated in Voronezh in 1918. Yuryev (primary – Derpt) University, founded in 1802 by the decree of the Russian emperor Alexander I in Derpt (later - Yuryev, nowadays Tartu), was the second Russian university. The University became the center of education, sciences and culture of Central Chernozem region and continues to carry out these functions now. Now the training of the students in Voronezh State University has been carried out at 17 faculties. The graduates of the University work in 90 countries of the world. The Department of Medical Biochemistry and Microbiology was open at VSU Biology and Soil Science Faculty in 1997. Working group of the Department (more than 10 people, from them 7 have scientific degrees) owns wide experience of work in the field of research of cell metabolism organization and regulation, enzymology. The results of the Department employees investigations lately have found the reflection in 7 candidate dissertations, tens publications in the central journals, materials of the International and Russian conferences on biological and medical subjects. The research work, spent on the Department, repeatedly was supported by the Russian and International grants.
Expertise offered Our team can bring contribution on the decision of biochemistry fundamental problem connected to research of functioning of antioxidant system (АОS) and oxidative metabolism enzymes, capable to limit free-radical oxidation (FRО), at pathologies, connected with oxidative stress (experimental models of liver toxic defeat, myocardium and brain ischemia, hyperthyroidism at rats), and also at action of substances-protectors (thioctic acid, citrate, melatonin and several synthetic substances, received at Voronezh State University Chemical Faculty). Estimation of free-radical processes intensity in analysed tissues is realised by biochemiluminescence method, giving the integral characteristic of a FRO level, definition of the contents of lipid peroxidation primary products – diene conjugates, and DNA fragmentation as index of apoptosis development degree. Activity of AOS enzymes (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase) and level of some antioxidants (α-tocopherol, citrate, glutathione) in liver, myocardium, brain and blood serum at pathologies and protectors action are investigated. The research of aconitase, citrate synthase, NAD- and NADP-isocitrate dehydrogenases, NAD- and NADP-malate dehydrogenases, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase functioning with application of purified enzyme preparations is also spent. The research of these enzymes expression regulation is realized. The reception of the information about properties of the enzymes capable to participate in a FRО intensity regulation in an organism by interaction, will allow to come nearer to comprehension of new aspects of antioxidant system enzymatic and not enzymatic components activity linking and regulation in mammals functionally various tissues at pathologies connected with oxidative stress development, and action of substances - protectors. The investigation of biochemical bases of reactive oxygen species formation regulation with the help of substances-protectors promotes clarification of molecular mechanisms of FRO intensity increase, which is a universal non-specific link in various diseases pathogenesis, prevention.
Scientific keywords Free-radical oxidation Antioxidant defense Oxidative metabolism enzymes Pathologies Substances-protectors
Publications on the topic (other references)
  1. T.N. Popova, T.I. Rakchmanova, K.-J. Appenroth. Cytosolic and chloroplastic NADP-dependent isocitrate dehydrogenase in Spirodela polyrhiza. Regulation of activity by metabolites in vitro // J. Plant Physiology. - 2002. - V.159. - P. 231-237.
  2. T.I. Rakhmanova, T.N. Popova. Regulation of 2 Oxoglutarate Metabolism in Rat Liver by NADP Isocitrate Dehydrogenase and Aspartate Aminotransferase // Biochemistry (Moscow). – 2006. -Vol.71, No.2. - P. 211 217.
  3. A.N. Pashkov, S.S. Popov, A.V. Semenikhina, T.I. Rakhmanova. Glutathione system state and activity of some NADPH-producing enzymes in rats liver under melatonin action at norm and toxic hepatitis // Bull. Exp. Biol. Med. (Moscow). – 2005. – V. 139, No. 5. – P. 520-524.
  4. A.N. Pashkov, S.S. Popov, A.V. Semenikhina, T.I. Rakhmanova. Oxidative status of rat serum blood at toxic damage of liver and melatonin action // J. Theoret. and Pract. Med. (Moscow). – 2006. – V. 4, No. 1. – P. 93-96.
  5. E.M. Andreescheva, T.N. Popova, V.G. Artyukhov, T.I. Rakhmanova, L.V. Matasova. The intensity of free radical oxidation and catalytic properties of rat liver NADP-isocitrate dehydrogenase in the norm and toxic hepatitis // Biomed. Chemistry (Moscow). – 2006. – V. 52, No. 2. – P. 153-160.
  6. M.M. Sviridov, A.V. Semenikhina, T.N. Popova. Catalytic properties of rat liver 6-phosphogluconate dehydrogenase in the norm and toxic hepatitis // Biomed. Chemistry (Moscow). – 2006. – V. 52, No. 5. – P. 479-488.
  7. O.A. Safonova, T.N. Popova, V.G. Artyukhov, L.V. Matasova. Cytoplasmic NAD-malate dehydrogenase functioning peculiarities in rat myocardium at ischemia conditions // Bull. Exp. Biol. and Med. (Moscow). – 2005. - V. 140, No. 7. – P. 31-34.
  8. O.A. Safonova, T.N. Popova, V.G. Artyukhov, L.V. Matasova. Free radical oxidation intensity and regulation of NADP-dependent malate dehydrogenase activity in rat cardiomyocytes in norm and under ischemia // Biomed. Chemistry (Moscow). – 2005. – V. 51, No. 3. – P. 311-321.
  9. O.A. Safonova, T.N. Popova, V.G. Artyukhov, L.V. Matasova. Oxidative status and functioning of NAD-dependent malate dehydrogenase in mitochondria of rat cardiomyocytes in norm and under ischemia // Voprosi Biol., Med. and Pharm. Chemistry (Moscow). – 2005. – No. 1. – P. 50-53.
  10. L.V. Matasova, A.V. Semenikhina, T.N. Popova. The influence of ascorbate and iron joint reception on free radical processes intensity in stomach and intestines tissues of rats // Voprosi Biol., Med. and Pharm. Chemistry (Moscow). – 2005. – No. 2. – P. 9-13.
  11. A.N. Pashkov, S.S. Popov, A.V. Semenikhina, L.V. Matasova, T.N. Popova. Melatonin influence on oxidative status, citrate content and aconitate hydratase activity in rat liver under toxic hepatitis // Probl. of Endocrinol. (Moscow). – 2005. – V. 51, No. 6. – P. 41-43.
  12. T. Popova, M.A.A. Pinheiro de Carvalho, L. Matasova, L. Medvedeva. Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia / Mol. and Cell. Biochem. - 2007. – Vol. 294. – P. 97-105. – DOI: 10.1007/s11010-006-9249-9. Article on-line: 2006. (http://www.springerlink.com/(xdfdun55stfvfcfd2zikvxyk)/app/home/contribution.asp).
Description of previous and present experience in International Cooperation -
Previous participation in EU’s Framework Programme projects