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Voronezh State University - KBBE-2008-3-2-01: Molecular modelling for rational design of industrial enzymes - Development of the heterogeneous biocatalyst for fructose production on the bases of vegetative inulin containing material

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Date of filling in 19.12.2007
Organization name Voronezh State University
Organization adress Universitetskaya pl., 1, Voronezh, 304006, Russia
Department/Unit Biological faculty, Chair of Biophysics and biotechnology
Contact person Kovaleva Tamara, DSc, Prof.
Phone +7-4732-208586; 208578
Fax +7-4732-208308
E-Mail tamara_kovaleva@inbox.ru
Web-site http://www.vsu.ru
Organization type
  • Research
  • Education
Special Programme
Theme
Food, Agriculture and Fisheries, and Biotechnology (KBBE)
Call identifier
Topic number KBBE-2008-3-2-01: Molecular modelling for rational design of industrial enzymes - Development of the heterogeneous biocatalyst for fructose production on the bases of vegetative inulin containing material
Call Deadline
Short description of the organization (max 12 lines): -
Expertise offered It is realized immobilization of enzyme inulinase on different supports. Using the methods of gel-chromatography, electrophoresis, infrared spectroscopy, atomic force microscopy and computer modeling it is established the spatial structure of inulinase. It is investigated physicochemical properties and kinetic-thermodynamic aspects for hydrolytical reaction of fructose containing polymer substrates.
Problems to be solved and results: It is planed to forget structure-functional inulinase models and creating on this bases heterogeneous biocatalysists in tubular reactor systems for fructose production from vegetative material.
Scientific keywords Inulinase Inuline Fructose Heterogeneous biocatalysists Immobilization
Publications on the topic (other references)
  • Kinetic and thermodynamic aspects of polysaccharide hydrolysis by native and immobilized amylases // T.A. Kovaleva – Biophysics. – 2000. – Vol. 45, № 3. – P. 427-432.
  • Spatial structure computer analysis of hydrolytic enzymes // V.G. Artyukhov, T.A. Kovaleva, O.M. Kozhokina, L.A. Bitutskaya, R.V. Dronov, O.D. Trofimova – Biochemistry. – 2005. – Vol. 70, № 10. – P. 1318-1327.
  • Development of the heterogeneous biocatalyst for reaction of inulin hydrolysis on basis of immobilized inulinase preparation from Kluyveromyces marxianus // T.A. Kovaleva, M.G. Holyavka, A.S. Taha – Biotechnology. – 2007. - № 3. - С. 80-87.
  • Influence of urea, γ- and UV-radiation on physicochemical properties of the native and immobilized inulinase // T.A. Kovaleva, O.M. Kozhokina, O.D. Trofimova. – Radibiology. Radioecology. – 2000. - т. 40, №1. - С. 23-27.
  • Influence of UV irradiation on physicochemical properties of immobilized inulinase Aspergillus awamori // T.A. Kovaleva, O.D. Trofimova, O.M. Kozhokina, A.S. Taha - 8th IUBMB Conference. –Boston. - 2004. - p. 17.
  • Investigation of kinetik and thermodynamic aspects of polymeric substrate hyrolisis by soluble and immobilization hydrolase // V.G. Artyukhov, T.A. Kovaleva, O.D. Trofimova, O.M. Kozhokina, A.S. Taha - Al-Mustansiria Journal of Science. - 2004. - №6. – P. 183-188.
Description of previous and present experience in International Cooperation -
Previous participation in EU’s Framework Programme projects