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Voronezh State University - HEALTH-2007-3.5-1: Epidemiological investigations into long-term trends of population health as consequence of socio-economic transitions, including life-style induced health problems - Research of structural and functional state of DNA and antioxidant status in mammals myocardium at action of substances – potential precursors of medical products - under pathologies, associated with oxidative stress

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Date of filling in 20.07.2007
Organization name Voronezh State University
Organization adress Universitetskaya pl., 1, Voronezh, 304006, Russia
Department/Unit Biology and Soil Science Faculty, Chair of Analytical and Medical Biochemistry and Microbiology
Contact person Popova Tatyana, DSc, Professor, Head of Chair
Phone +7-4732-208278
Fax +7-4732-208755
E-Mail tpopova@bio.vsu.ru
Web-site http://www.vsu.ru
Organization type
  • Research
  • Education
Special Programme
Theme
Health
Call identifier
Topic number HEALTH-2007-3.5-1: Epidemiological investigations into long-term trends of population health as consequence of socio-economic transitions, including life-style induced health problems - Research of structural and functional state of DNA and antioxidant status in mammals myocardium at action of substances – potential precursors of medical products - under pathologies, associated with oxidative stress
Call Deadline
Short description of the organization (max 12 lines): -
Expertise offered The activity is directed on the decision of problems of biochemistry, medicine and pharmacology, connected to investigation of the biochemical bases of reactive oxygen species formation regulation with the help of substances-protectors and development of cardiovascular system various pathologies early diagnostics methods, search and introduction to a clinical practice of new highly effective medical products of various purpose. Research of structural and functional state of DNA and functioning of antioxidant system (АОS) and oxidative metabolism enzymes, capable to limit free-radical oxidation (FRО), in animals heart at pathologies, connected with oxidative stress (experimental models of myocardium ischemia, hyperthyroidism, apoptosis induction by tumor necrosis factor α at rats), and also at action of substances-protectors (thioctic acid, citrate, melatonin and several synthetic substances, received at Chemical Faculty of Voronezh State University) is carried out. As an index of apoptosis development degree the method of estimation of DNA, extracted from analysed samples, fragmentation is used. Estimation of free-radical processes intensity is realised by biochemiluminescence method and definition of the contents of lipid peroxidation primary products. Activity of AOS enzymes (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase) and level of some antioxidants (α-tocopherol, citrate, glutathione) in myocardium and blood serum at pathologies and protectors action are investigated. The research of aconitase (АH), NAD- and NADP-isocitrate dehydrogenases (NAD-IDH and NADP-IDH), NAD- and NADP-malate dehydrogenases (NAD-MDH and NADP-MDH) functioning with application of purified enzyme preparations is also spent. The research of these enzymes expression regulation in tissues of animals of experimental groups has been begun.
Problems to be solved and results:
  1. Research of extracted from heart of animals DNA fragmentation degree at pathology and action of substances - potential precursors of medical products.
  2. Estimation of FRO intensity in rats heart and blood serum at myocardium ischemia, hyperthyroidism, apoptosis induction by tumor necrosis factor α and also at introduction of substances-protectors.
  3. Definition of AOS components activity at pathological conditions and at introduction of protectors (melatonin, thioctic acid, citrate, several synthetic substances).
  4. Definition of activity of some central metabolic pathways enzymes (АH, NAD- and NADP-IDH, NAD- and NADP-MDH) in conditions of a pathology and at action of substances-protectors, and research of these enzymes properties with purified enzyme preparations use.
  5. Study of peculiarities of oxidative metabolism enzymes expression and clarification of their induction and repression possibility at oxidative stress development and protectors action.
Scientific keywords Free-radical oxidation Structural and functional state of DNA Antioxidant defense Oxidative metabolism enzymes Rats Myocardium ischemia Hyperthyroidism Tumor necrosis factor α Substances-protectors
Publications on the topic (other references)
  • Medvedeva L. V., M.A.A.Pinheiro de Carvalho, T.N.Popova, Artujkhov V. G., Matasova L.V.The influence of Phentone reaction participants and glutatione on NADP-specific isocitric dehydrogenase of rat cardiomyocites at normal and ishemical conditious // Proceedings of the 27th Meeting of the Federation of European Biochemical Societies. Lisbon. Portugal. 30 June-5 July 2001. European J.Biochemistry. Special issue. P.165. Abstr. PS 6-136.
  • Shikhaliev Kh.S. Hetarylguanidines in reactions of heterocyclization / Kh.S. Shikhaliev [et al.] // Nitrogen-containing heterocycles and alkaloids. Editors: V.G. Kartsev, G.A. Tolstikov. - M.: Iridium Press, 2001. - V. 1. - P. 498-503.
  • Popova T., Pinheiro de Carvalho M.A.A., Medvedeva L., Matasova L. Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia / Mol. and Cell. Biochem. – 2007. – 294:97-105, DOI: 10.1007/s11010-006-9249-9 [IF=1,681] (http://www.springerlink.com/(xdfdun55stfvfcfd2zikvxyk)/app/home/contribution.asp).
Description of previous and present experience in International Cooperation -
Previous participation in EU’s Framework Programme projects