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Yerevan State University - Research of the oxidative status and oxidative metabolism enzymes activity in mammals tissues at pathologies, connected with oxidative stress, and protectors action

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Date of filling in 16.03.2007
Organization name Yerevan State University
Organization adress Universitetskaya pl., 1, Voronezh, 304006, Russia
Department/Unit Biology and Soil Science Faculty, Chair of Analytical and Medical Biochemistry and Microbiology
Contact person Popova Tatyana, DSc, Professor, Head of Chair
Phone +7-4732-208278
Fax +7-4732-208755
E-Mail tpopova@bio.vsu.ru
Web-site http://www.vsu.ru
Organization type
  • Research
  • Education
Special Programme
Theme
Health
Call identifier
Topic number Research of the oxidative status and oxidative metabolism enzymes activity in mammals tissues at pathologies, connected with oxidative stress, and protectors action
Call Deadline
Short description of the organization (max 12 lines): -
Expertise offered The activity is directed on the decision of a fundamental problem of biochemistry connected to research of functioning of antioxidant system (АОS) and oxidative metabolism enzymes, capable to limit free-radical oxidation (FRО), at pathologies, connected with oxidative stress (experimental models of liver toxic defeat, myocardium and brain ischemia, hyperthyroidism at rats), and also at action of substances-protectors (thioctic acid, citrate, melatonin and several synthetic substances, received at Voronezh State University Chemical Faculty). Estimation of free-radical processes intensity in analysed tissues is realised by biochemiluminescence method, giving the integral characteristic of a FRO level, definition of the contents of lipid peroxidation primary products – diene conjugates, and DNA fragmentation as index of apoptosis development degree. Activity of AOS enzymes (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase) and level of some antioxidants (-tocopherol, citrate, glutathione) in liver, myocardium, brain and blood serum at pathologies and protectors action are investigated. The research of aconitase (АH), succinate dehydrogenase (SDH), citrate synthase (CS), NAD- and NADP-isocitrate dehydrogenases (NAD-IDH and NADP-IDH), NAD- and NADP-malate dehydrogenases (NAD-MDH and NADP-MDH), glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) functioning with application of purified enzyme preparations is also spent. The research of these enzymes expression and repression regulation is planed. The investigation of biochemical bases of reactive oxygen species formation regulation with the help of substances-protectors promotes clarification of molecular mechanisms of FRO intensity increase, which is a universal non-specific link in various diseases pathogenesis, prevention.
Problems to be solved and results:
  1. Estimation of FRO intensity in rats liver, heart, brain and blood serum at an experimental toxic hepatitis, myocardium infarction, brain ischemia and hyperthyroidism and also at introduction of substances-protectors.
  2. Definition of AOS components activity at pathological conditions and at introduction of protectors (melatonin, thioctic acid, citrate, several synthetic substances).
  3. Definition of activity of some central metabolic pathways enzymes (CS, АH, NAD- and NADP-IDH, SDH, NAD- and NADP-MDH, G6PDH, 6PGDH) in conditions of a pathology and at action of substances-protectors, and research of these enzymes properties with purified enzyme preparations use.
  4. Study of peculiarities of oxidative metabolism enzymes expression and clarification of their induction and repression possibility at oxidative stress development and protectors action.
Scientific keywords Free-radical oxidation Antioxidant defense Oxidative metabolism enzymes Rats Toxic hepatitis Myocardium infarction Brain ischemia Hyperthyroidism Substances-protectors
Publications on the topic (other references) -
Description of previous and present experience in International Cooperation -
Previous participation in EU’s Framework Programme projects