Voronezh State University
Popova Tatyana N.
Head of chair, D.Sc., professor.
Offers of high school on participation in joint researches with the European universities within the limits of thematic priorities of the Seventh frame program of scientifically-technological development of EU
Cooperation / Health / Optimising the delivery of health care to European citizens
HEALTH-2007-2.4.2-7 Area: Research of structural and functional state of DNA and antioxidant status in mammals myocardium at action of substances – potential precursors of medical products - under pathologies, associated with oxidative stress
The brief description of the project: The activity is directed on the decision of problems of biochemistry, medicine and pharmacology, connected to investigation of the biochemical bases of reactive oxygen species formation regulation with the help of substances-protectors and development of cardiovascular system various pathologies early diagnostics methods, search and introduction to a clinical practice of new highly effective medical products of various purpose. Research of structural and functional state of DNA and functioning of antioxidant system (АОS) and oxidative metabolism enzymes, capable to limit free-radical oxidation (FRО), in animals heart at pathologies, connected with oxidative stress (experimental models of myocardium ischemia, hyperthyroidism, apoptosis induction by tumor necrosis factor alfa at rats), and also at action of substances-protectors (thioctic acid, citrate, melatonin and several synthetic substances, received at Chemical Faculty of Voronezh State University) is carried out. As an index of apoptosis development degree the method of estimation of DNA, extracted from analysed samples, fragmentation is used. Estimation of free-radical processes intensity is realised by biochemiluminescence method and definition of the contents of lipid peroxidation primary products. Activity of AOS enzymes (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase) and level of some antioxidants (-tocopherol, citrate, glutathione) in myocardium and blood serum at pathologies and protectors action are investigated. The research of aconitase (АH), NAD- and NADP-isocitrate dehydrogenases (NAD-IDH and NADP-IDH), NAD- and NADP-malate dehydrogenases (NAD-MDH and NADP-MDH) functioning with application of purified enzyme preparations is also spent. The research of these enzymes expression regulation in tissues of animals of experimental groups has been begun
Expected results: Research of extracted from heart of animals DNA fragmentation degree at pathology and action of substances - potential precursors of medical products; Estimation of FRO intensity in rats heart and blood serum at myocardium ischemia, hyperthyroidism, apoptosis induction by tumor necrosis factor alfa and also at introduction of substances-protectors; Definition of AOS components activity at pathological conditions and at introduction of protectors (melatonin, thioctic acid, citrate, several synthetic substances); Definition of activity of some central metabolic pathways enzymes (АH, NAD- and NADP-IDH, NAD- and NADP-MDH) in conditions of a pathology and at action of substances-protectors, and research of these enzymes properties with purified enzyme preparations use; Study of peculiarities of oxidative metabolism enzymes expression and clarification of their induction and repression possibility at oxidative stress development and protectors action
The countries of partners under the project: Austria, Belgium, Bulgaria, United Kingdom, Hungary, Germany, Greece, Denmark, Spain, Italy, Netherlands, Poland, Portugal, Romania, Slovakia, Finland, Czech republic, Switzerland, Sweden
The information on the organization
Voronezh State University
CBS RIP, 478а, VSU, 1 Universitetskaya pl.