Voronezh State University
Popova Tatyana N.
Head of chair, D.Sc., professor.
Offers of high school on participation in joint researches with the European universities within the limits of thematic priorities of the Seventh frame program of scientifically-technological development of EU
Cooperation / Health / Translating research for human health / Other actions across the Health Theme
Research of the oxidative status and oxidative metabolism enzymes activity in mammals tissues at pathologies, connected with oxidative stress, and protectors action
The brief description of the project: The activity is directed on the decision of a fundamental problem of biochemistry connected to research of functioning of antioxidant system (АОS) and oxidative metabolism enzymes, capable to limit free-radical oxidation (FRО), at pathologies, connected with oxidative stress (experimental models of liver toxic defeat, myocardium and brain ischemia, hyperthyroidism at rats), and also at action of substances-protectors (thioctic acid, citrate, melatonin and several synthetic substances, received at Voronezh State University Chemical Faculty). Estimation of free-radical processes intensity in analysed tissues is realised by biochemiluminescence method, giving the integral characteristic of a FRO level, definition of the contents of lipid peroxidation primary products – diene conjugates, and DNA fragmentation as index of apoptosis development degree. Activity of AOS enzymes (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase) and level of some antioxidants (alfa -tocopherol, citrate, glutathione) in liver, myocardium, brain and blood serum at pathologies and protectors action are investigated. The research of aconitase (АH), succinate dehydrogenase (SDH), citrate synthase (CS), NAD- and NADP-isocitrate dehydrogenases (NAD-IDH and NADP-IDH), NAD- and NADP-malate dehydrogenases (NAD-MDH and NADP-MDH), glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) functioning with application of purified enzyme preparations is also spent. The research of these enzymes expression and repression regulation is planed.
The investigation of biochemical bases of reactive oxygen species formation regulation with the help of substances-protectors promotes clarification of molecular mechanisms of FRO intensity increase, which is a universal non-specific link in various diseases pathogenesis, prevention.
Expected results: Estimation of FRO intensity in rats liver, heart, brain and blood serum at an experimental toxic hepatitis, myocardium infarction, brain ischemia and hyperthyroidism and also at introduction of substances-protectors; Definition of AOS components activity at pathological conditions and at introduction of protectors (melatonin, thioctic acid, citrate, several synthetic substances); Definition of activity of some central metabolic pathways enzymes (CS, АH, NAD- and NADP-IDH, SDH, NAD- and NADP-MDH, G6PDH, 6PGDH) in conditions of a pathology and at action of substances-protectors, and research of these enzymes properties with purified enzyme preparations use; Study of peculiarities of oxidative metabolism enzymes expression and clarification of their induction and repression possibility at oxidative stress development and protectors action
The countries of partners under the project: Germany, Spain, Portugal, France, Sweden, United Kingdom
The information on the organization
Voronezh State University
CBS RIP, 478а, VSU, 1 Universitetskaya pl.